Alberta NINT/13 June 2008


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lab work (SD):

            Using the transformation completed by Wayne (12/06/07) of XL1-B cells with I732018 and the overnight
            cultures, DNA was isolated by QIAprep standard miniprep protocol.
            I732018 was sequenced.
            I732018 was digested with XbaI + PstI.  No evidence of DNA in the gel.  Retry!
            NINT SD p37.jpg

lab work (JD):

            Quantitated K102005/BamH1 and Nco1
            Ligated K102005/BamH1 and Nco1 with TA0/BamH1 and Nco1 (1/100 dilution) to create plasmid K102007
            Transformed XL1-B cells with K102007 and negative control
            Plated cells and left them for O/N