Alberta NINT/20 June 2008


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lab work (SD):

            Digested K102002.1-.6 and freshly annealed TA0 with BamHI + NcoI.
            Incubated at 37 C for 2 hrs.
            Ran K102002.1-.6 on 2% agarose gel at 110V for 50 min.
            Purified TA0 using QIAquick nucleotide removal protocol.
            Quantified DNA for K102002 and TA0.
            Ligated TA0 into K102002.1-.6 and incubated at 16 C for 30 min.
            NINT SD p46.jpg

lab work (JD):

             Digested K102005 with BamH1 + NcoI and incubated for 2 hrs at 37C
             Ran on 2% agarose gel slowly at 90V
             Cut out desired fragment and gel purified