Brown: Team Resistance/21 July 2008


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21 July 2008

  • Weekly Update Meeting: Suggestions included - Centrifuge bacteria before and after lysis and take readings of the supernatant. The alternating current we are currently using could be causing electroporation of the cells. Electroporation would cause the cells to open and close repeatedly. Consider using voltage sensitive dyes to verify lysis.
  • To determine sensitivity, we will need to make serial dilutions to see where a change can be detected. Once that is found, we can use that as a starting base and add our bacteria to that specific molar solution and a change will hopefully be seen. This would involve resuspending our cells in water mixed with NaCl.