Brown: Team Resistance/27 October 2008


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October 27, 2008

  • Another miniprep of the S105 gene transformation performed.
  • Optical Density Measurement: 71.6 ng/ul
  • Restriction Digest Verification done of S105 gene with restriction enzymes EcoRI and PstI (Buffer H). Placed in thermocycler for 1 hour at 37 degrees and heat shock 20 minutes at 80 deg C.

  • Performed another ligation of PVJ4 and pSB1AK3.
  • We did see growth for the transformations of pVJ4 and S105 Cassette!! Cultures were placed in the incubator overnight for miniprepping tomorrow.
  • Optical Density and conductance test run again with little success. Solution is not clearing up as expected. Cultures will be made from the glycerol stock plates (Colonies 2,3, and 4).