EPF-Lausanne/6 October 2008

From 2008.igem.org

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Cloning

Agarose Gel: Clones of LuxI with RBS and Term. added by 2Step PCR

Results of the plating of RBS-LuxI-Term:

40 colonies, and 6 on the vector autoligation.

  • 8 colonies picked and miniprep'd.
  • Ctrl. digestion with X,P.

Expected bands:

  • 2.1kb (vector pSB1A2)
  • 800bp (luxI by 2step pcr)

Clones 2, 3, 5 and 8 are correct.

Cloning of A1 into B1:

Cloning was succesful.