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Edinburgh/10 August 2008
From 2008.igem.org
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Week 8
Sunday 10 August 08
- Extracted C. fimi genomic DNA, followed by PCR of rrnB (P61), cenA (P62) and cex (P63) from genomic DNA. (CF)
- Transformed competent cells with L36, pSB1A2+crtY (Plates 104-105) and L37, pSB1A2+rbs+crtY (Plates 106-107). (CF)
- Set-up cultures from plates 102 (pSB1A2+crtB+crtI) and 103 (pSB1A2+glgC-mut1,2) for miniprep and from plates 74 (pSB1A2+rbs+dxs, as for M72) and 83 (pSB1A2P+rbs+appY, as for M82) for maxipreps. (CF)
