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Edinburgh/17 September 2008
From 2008.igem.org
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Week 14
Wednesday 17 September 08
- Sent M211-212 (pSB1A2-glgC16), M215 (pSB1A2-bglX) for sequencing. (AM)
- P97: Third attempt at MABEL PCR for cex using M150 as template with 10µl 50% glycerol, denaturing 1 min, annealing 58°C, extension 95 sec. (AM)
- Re-run M213, M217-M221 (dxs+crtEBI) on Gel 79, results looked promising and M213 and M218 will be sent for sequencing tomorrow. (YAN)
- Decided to give up on cenA and use CHU1727 endoglucanase from Cytophaga instead. CKL (CF's PhD student) is working on this. (AM)
- Update: CF continued work on cenA, eventually obtaining a valid BioBrick.
