Edinburgh/18 September 2008

From 2008.igem.org

< Previous Entry | Notebook Index | Next Entry >

Week 14

Thursday 18 September ~ Friday 19 September 08

  • Sent M213, M218 (dxs+crtEBI) for sequencing. (AM)
  • Single (EcoRI) and double (EcoRI+PstI) digests of:
    • M198 (SOB2+SU1)
    • M201~203 (SOB2-rbs+SU1)
    • M204~206 (SOB2+ISO2)
    • M207~209 (SOB2-rbs+ISO2). (Yan)
  • Single (PstI) digest of P97 (cex mut). (AM)
  • M198~M206 run on Gel 80. All failed: single and double digests yielded the same results for all samples. (Yan)
  • M207~209 and P97 run on Gel 81. All failed: single and double digests yielded the same results for M207~209. PstI digest of P97 resulted in a smear. (AM)
  • Transformation/plating of CHU1727 failed twice. (CKL)

Follow-Up: Sequencing Results

  • M212 is pSB1A2-glgC16 with desired prefix and suffix.
  • M213 is NOT dxs+crtEBI. Both forward and reverse sequences indicate crtI.
  • M218 is NOT dxs+crtEBI. Forward sequence is dxs; reverse sequence is gibberish.