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Edinburgh/19 July 2008
From 2008.igem.org
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Week 5
Saturday 19 July 08
- Minipreps M43 to M47 of possible glgC double mutants (both EcoRI sites gone), and M48 of the only white colony from the pSB1A2+rbs+dxs transformation. Gel 18 shows EcoRI/PstI digests of 3μl. M43 to M47 all look about right: send one for sequencing. M48 is ambiguous, could be incomplete deigestion, need to repeat. (CF)
- PCR P24 and P25, repeat PCR for cenA and cex (the two shorter genes) using Pfu polymerase instead of KOD, adding 5% glycerol - this worked well for high-GC genes from Saccharothrix espanaensis last year. (CF)
