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Edinburgh/9 July 2008
From 2008.igem.org
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Week 4
Wednesday 9 July 08
- Results of L6 (BABEL2+glgC-mut1) and L7 (BABEL2+glgC-mut2) transformations: no colonies for L6, but 9 colonies were obtained from L7. These were subcultured to plates 26 and 27. By the end of the day, these had grown sufficiently for 6 overnight cultures to be set up for minipreps tomorrow. In theory, some or all of these should be glgC with EcoRI site 2 mutated out.
- Minipreps M13 to M18 of possible pSB1A2+appY transformants. Digests with EcoRI/PstI were run on Gel 10. All but M13 looked OK (insert band on M14 was so faint as to be ambiguous, but M15 to M18 all look good).
- Digests and ligation L8 for addition of a synthetic ribosome binding site M2 (which has now been entered into the Registry as BBa_K118000).
