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File:Jr pulsegen 5.jpg
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Pulse generator construct K142027 was produced from device K142047 using PCR-based site-directed mutagenesis as described previously. After transformation by electroporation of purified PCR product into DH5-alpha cells, eight colonies were selected and plasmids were purified using Qiagen Miniprep kit. The eight plasmids have been co-transformed together with target plasmid K142073 into electrocompetent DH5-alpha cells and were plated on LB-agar supplemented with carbenicillin and kanamycin. Upon incubation overnight at 37C, numerous colonies appeared and one colony from each plate was streaked onto a LB-agar plate supplemented with carbenicillin and kanamycin and on a LB-agar plate supplemented with carbenicillin, kanamycin and 1mM IPTG. Cells were incubated overnight at 37C for the same duration and imaged with a Nikon D80 SLR on the UV lamp of a Kodak Gel Logic 200 imaging system.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 00:48, 30 October 2008 | | 821×469 (215 KB) | Jrabl (Talk | contribs) | (Pulse generator construct [http://partsregistry.org/wiki/index.php?title=Part:BBa_K142027 K142027] was produced from device [http://partsregistry.org/wiki/index.php?title=Part:BBa_K142047 K142047] using PCR-based site-directed mutagenesis as described pr) |
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