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Illinois/17 July 2008
From 2008.igem.org
Antibody/GPCR Fusion
- Consulting meeting with Huimin Zhao (Associate Professor, Chemical and Biomolecular Engineering)
LAB WORK
Receptor Tyrosine Kinase method
E.Coli Media
LB medium, per liter
10g tryptone
5g yeast extract
5g NaCl
1 mL 1N NaOH
15g (agar for plates)
1. antibody DNA resuspended in TE buffer, 0.1 μg/μL
2. 5mL LB inoculated with single colony DH5a pro
3. Incubated at 37 degrees overnight
