27 August 2008

Wetlab

• Today a Midi prep of CAT and the B.subtilis vectors pDR110 and pDR111 were performed. Validation of the DNA was carried out by running a gel electrophoresis of the DNA.

Lanes : M = Marker, 1 = CAT Midi 1 Digested, 2 =CAT Midi 2 Digested, 3 = pDR110 Midi 1 Undigested, 4 = pDR110 Midi 2 Undigested, 5 = pDR111 Midi 1 Undigested, 6 = pDR111 Midi 2 Undigested

• Both the plasmids and the CAT insert appear to be the correct size and contamination appears to be fairly negligible

Dry Lab

• Obtained a second better quality microscope video of B. Subtilis swimming (better contrast, better focus...)
• Failed to track the cells using Spot Tracker plugin in ImageJ. More investigation needed.