9 October 2008

Wetlab

• The ligations of various parts were transformed and single colony PCR was carried out to check for correct ligation. The following parts that were ligated were:
  • 3'AmyE + GFP-T
  • 3'AmyE + RFP-T
  • 6L(LipA-Elastin)
• The pSB primers were used on the following cycle:
  • 1 cycle - 95^oC for 30 sec
  • 30 cycle - 95^oC for 30 sec, 60^oC for 45 sec, 72^oC for 1 min

• All ligations appear to have worked and now midi preps will be prepared.