TUDelft/22 October 2008

From 2008.igem.org

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October 22nd

Luciferase & Protein Content: Bead Beater style

Today we've done a new luciferase assay using the beadbeater. Results of BCA (protein content) are displayed in figure 1.

Figure 1: Total protein content (BCA) measurement of samples treated with lysozyme and beadbeater


Corrected luciferase measurements are displayed in figure 2.

Figure 2: Protein content corrected luciferase measurement of samples treated with lysozyme and beadbeater

We've done some luciferase measurements also using the old protocol with lysis buffer. These are displayed in figure 3, but aren't corrected for protein content but for OD.

Figure 3: OD corrected luciferase measurement of samples treated with Promega Luciferase Lysis Buffer

Miniprep

Three different vectors were prepped with a Qiagen kit according to manufacturer's protocol. These three didn't yield a sufficient DNA concentration yesterday. Today after prepping these were fine and so all vectors are ready to be sequenced and also ready to be sent to Boston.