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Team:BCCS-Bristol/Calendar-Notebook/22 July 2008
From 2008.igem.org
Bead experiment 1
It was started using the beads, aspartate and bacteria in 0.3 % agar within the glass slide chambers. One bead was focused under the microscope; it didn't move after 30 minutes probably due to the observation that the bacteria were in the bottom layer and the beads on top of the motility medium in didn`t seem to get in contact. The chamber was left under the microscope overnight (results see 23 July 2008).
BioBrick Transformation
Carried on with biobrick transformation of the GFP generator. The DNA was transformed into E. coli DH5α cells along with pUC19 as a control using chemical competent cells and heat shock.
Swimming agar assay
The chemotaxis experiment was continued, set up gradient over 8 hours of diffusion and innoculated with both MC1000 and MG1655 in agar in wells as before ( 21 July 2008).
