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Testing different volumes of AHL
Our previous experiments were inconclusive because we believed that we possibly swamped our Bacillus with excess amounts of AHL so that no appreciable degradation was seen. In this experiment we tested and compared different concentrations of AHL.
Fluorescence with 1 uM AHL
Fluorescence with 10nM AHL
Fluorescence with 1nM AHL
- not enough for good readings
Images of 1uM AHL plates with accompanying plot profiles
The graphs demonstrate fluorescence change across the plate from left to right.
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