Team:Chiba/Calendar-Home/10 September 2008

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9 September 2008 <|> 11 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(9/9)-->Colony Count

  1. LuxI(BBa_K084012)
  2. LuxI+LVA(BBa_K084014)
  3. LasI(BBa_K084007)
  4. Background(R0010)
  5. Background(BBa_C0261)

Pick colony

Colony-PCR

Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate(BBa_F2620(2007)).
DNA Template(μL) 1
dNTP mix(μL) 5
Foward Primer(μL) 0.3
Reverse Primer(μL) 0.3
taq DNA polymerase(μL) 0.5
Thermopol Buffer(μL) 3
Nuclease free water(μL) 20
TOTAL(μL) 30


95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C


-->Gel Check

Chiba-0910.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μL
From left;
Marker
  1. BBa_K084007 -> OK
  2. BBa_K084007 -> OK
  3. BBa_K084007 -> OK
  4. BBa_K084007 -> OK
  5. BBa_K084007 -> OK
  6. BBa_K084007 -> OK
  7. BBa_K084007 -> Bad
  8. BBa_K084007 -> OK
  9. BBa_K084007 -> OK
  10. BBa_K084007 -> OK
Marker
  1. BBa_K084014 -> OK
  2. BBa_K084014 -> OK
  3. BBa_K084014 -> OK
  4. BBa_F2620 ---control -> OK
Marker


--->(28/8)Miniprep

eluted with 50μL of TE buffer.

Team:Output

TIME Responce(liquid)

TIME Responce(solid)

Colony PCR

-->Gel check

-->Mini prep

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