"
Team:Chiba/Calendar-Home/13 September 2008
From 2008.igem.org
12 September 2008 <|> 14 September 2008
Contents |
Laboratory work
Team:Input
Time Delay Test
- time-delay test
- transformants
receiver
- BBa_T9002(JW1908)
sender
- Plac-RhlI (JW1908)-Amp
- Plac-LasI (JW1908)-Amp
- Ptet-LuxI (JW1908)-Amp
48-well plate(Deep Well)
| 1:2ml | 2:2ml,AHL | 2:2ml | 3:2ml | 4:2ml | - |
| - | - | - | - | - | - |
| 1:1ml,2:1ml,IPTG:2μl | 1:100μl,2:1ml,IPTG:1.1μl | 110μl,21ml,IPTG:101μl | 1:1μl,21ml,IPTG:1.001μl | 1:1ml,2:100μl,IPTG:1.1μl | 1:1ml,2:10μl,IPTG:1.01μl |
| - | - | - | - | - | - |
| 1:1ml,3:1ml,IPTG:2μl | 1:100μl,3:1ml,IPTG:1.1μl | 1:10μl,3:1ml,IPTG:1.01μl | 1:1μl,3:1ml,IPTG | 1:1ml,3:100μl,IPTG:1.1μl | 1:1ml,3:10μl,IPTG:1.01μl |
| - | - | - | - | - | - |
| 1:1ml,4:1ml,IPTG:2μl | 1:100μl,4:1ml,IPTG:1.1μl | 1:10μl,4:1ml,IPTG:1.0μl | 1:1μl,4:1ml,IPTG:1.001μl | 1:1ml,4:100μl,IPTG:1.0μl | 1:1ml,4:10μl,IPTG1.01μl |
| - | - | - | - | - | - |
Team:Communication
- Gel Check
- BBa_C0161(2007)
- BBa_R0010(2007)
- BBa_J04500(2007)
|
|
-->Digestion
- BBa_C0161(2007)
- BBa_J04500(2007)
- BBa_R0010(2007)
| Sample No. | 1 | 2 | 3 | 4 |
| Sample DNA(μL) | 5 | 5 | 1 | 1 |
| EcoRI(μL) | 0.5 | o.5 | - | - |
| SpeI(μL) | 0.5 | 0.5 | - | - |
| Buffer 1(μL) | 1 | 1 | - | - |
| Buffer 3(μL) | - | - | 1 | - |
| BSA(μL) | 1 | 1 | - | - |
| dH2O(μL) | 2 | 2 | 7.5 | - |
| TOTAL(μL) | 10 | 10 | 10 | 1 |
--->(9/14)[[1]]
Team:Output
no work
