Team:Chiba/Calendar-Home/29 August 2008

From 2008.igem.org

>Home | Notebook

28 August 2008 <|> 30 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

--->Digestion Test
  1. BBa_R0010(2007)
  2. BBa_C0062(2007)
  3. BBa_C0179(2007)
  4. BBa_C0179(2006)
  5. BBa_S03156(2007)
  6. BBa_S03156(2006)
  7. BBa_S03158(2007)
  8. BBa_S03158(2006)
  9. BBa_S03160(2007)
  10. BBa_S03160(2006)


Sample No. S1~10W1W2~10
Sample DNA 142
PstI -0.0750.1
EcoRI 0.10.0750.1
Buffer 3 10.751
BSA -0.751
dH2O 7.94.355.8
TOTAL 10μl10μl10μl


--->Gel Check
Chiba-0829.JPG
Sample No. 1~10S1~S10,W1~W10
Sample DNA 110
Loading Dye 12
TOTAL 6μl12μl
From left;
1~5,S1,S5,W1~W5
Chiba-0829-2.JPG
From left;
6~10,S6~S10,W6~W10


(27/8)--->Gel Check
Chiba-0829-3.JPG
Sample DNA 0.5
Loading Dye 1
dH2O 4.5
TOTAL 6μL
R0010 -> OK


Digestion

  1. BBa_I9026(2007)
  2. BBa_I9030(2007)
  3. BBa_S03154(2007)
  4. BBa_R0010(2006)


Sample No. 1~34
Sample DNA 100ng/μL×20200ng/μL×30
PstI 22
XbaI 2-
SpeI -2
Buffer 2 -5
Buffer 3 5-
BSA 55
dH2O 166
TOTAL 50μl50μl
--->Gel Check
Chiba-0829-4.JPG
Sample No. 1~34
Sample DNA 103
Loading Dye 22
dH2O -7
TOTAL 12μl12μl
insertBBa_I9026 : 685bp -> None
Chiba-0829-5.JPG
insertBBa_I9026  : 745bp -> None
Chiba-0829-6.JPG
insertBBa_S03154 : 685bp -> None
Chiba-0829-7.JPG
vectorBBa_R0010 : 2079bp -> OK


--->Gel Extract


--->Zymo Clean
->15μL
--->SAP
Sample No. 4
Sample DNA 15
SAP 2
SAP Buffer 3
dH2O 10
TOTAL 30μL


--->Zymo Clean
--->Suspension! (We got information, by the team-output, to the effect that the density of the sample is too low.)

Team:Output

TIME RESPONCE(solid)

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/29_August_2008"