Team:Chiba/Calendar-Home/31 August 2008
From 2008.igem.org
30 August 2008 <|> 1 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- Transformation
- Competent Cells : XL10Gold
- BBa_K084007(Plac+RBS+LasI)
- BBa_K084008(Plac+RBS+RhlI)
- ---> We saved these plates with a refrigerator.
- Digestion
- BBa_I9026(2007)
- BBa_I9030(2006)
- BBa_S03154(2007)
- BBa_R0010(2007)
Sample No. 1~3 4 Sample DNA(μL) 12 10 PstⅠ(μL) 0.2 0.2 XbaⅠ(μL) 0.2 - SpeⅠ(μL) - 0.4 Buffer 2(μL) - 1.5 Buffer 3(μL) 2 - BSA(μL) 2 1.5 dH2O(μL) 3.6 1.4 TOTAL(μL) 20 15
- --->(1/9)Gel Check
- (30/8)--->Mini prep
- --->Digestion test
- BBa_K084008 Sample No.2~5
- BBa_T9002(1),(2)
Sample Single Digesiton 2~5 Double Digestion 2~5 Single Digestion T9002(1) Single Digesiton T9002(2) Double Digestion T9002(1)(2) Sample DNA(μL) 1 3 3 1 3 XbaI(μL) 0.1 0.1 0.1 0.1 0.1 SpeI(μL) - 0.1 - - 0.1 Buffer 2(μL) 0.9 0.8 0.9 0.9 0.8 BSA(μL) 1 1 1 1 1 dH2O(μL) 7 5 5 7 5 TOTAL(μL) 10 10 10 10 10
- --->Gel Check
Sample No. BBa_K084008 Sample No.2~5, BBa_T9002(1)(2) Single & Double Digestion Sample DNA(μL) 1 10 Loading Dye(μL) 1 2 dH2O(μL) 4 - TOTAL(μL) 6 12 - From Left
- Single Digestion BBa_K084008 2~5
- Single Digestion BBa_T9002(1),(2)
- Double Digestion BBa_K084008 2~5
- Double Digestion BBa_T9002(1),(2)
- BBa_T9002(1),(2)
- From Left
Team:Output
- BBa_R0010(1)
- BBa_J52008(2)
Sample No. 1 2 DNA 10 10 SpeI 1 0 PstI 1 1 XbaI 0 1 BSA(x100) 0.5 0.5 Buffer2 5 0 Buffer3 0 5 dH2O 28.5 28.5 TOTAL 50μl 50μl -->left for 3 hours at 37 °C.
-->(1)20μl refine
-->(2)20μl refine