Team:Chiba/Calendar-Home/31 August 2008

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30 August 2008 <|> 1 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

Transformation
Competent Cells : XL10Gold
---> We saved these plates with a refrigerator.


Digestion
  1. BBa_I9026(2007)
  2. BBa_I9030(2006)
  3. BBa_S03154(2007)
  4. BBa_R0010(2007)
Sample No. 1~34
Sample DNA(μL)1210
PstⅠ(μL) 0.20.2
XbaⅠ(μL)0.2-
SpeⅠ(μL)-0.4
Buffer 2(μL) -1.5
Buffer 3(μL) 2-
BSA(μL) 21.5
dH2O(μL) 3.61.4
TOTAL(μL) 2015


--->(1/9)Gel Check


(30/8)--->Mini prep


--->Digestion test
Sample Single Digesiton 2~5Double Digestion 2~5Single Digestion T9002(1)Single Digesiton T9002(2)Double Digestion T9002(1)(2)
Sample DNA(μL)13313
XbaI(μL)0.10.10.10.10.1
SpeI(μL)-0.1--0.1
Buffer 2(μL) 0.90.80.90.90.8
BSA(μL) 11111
dH2O(μL) 75575
TOTAL(μL) 1010101010


--->Gel Check
Chiba-0831-p87.JPG
Sample No. BBa_K084008 Sample No.2~5, BBa_T9002(1)(2) Single & Double Digestion
Sample DNA(μL) 110
Loading Dye(μL) 12
dH2O(μL) 4-
TOTAL(μL) 612
From Left

Team:Output

Digestion

Sample No. 12
DNA 1010
SpeI 10
PstI 11
XbaI 01
BSA(x100) 0.50.5
Buffer2 50
Buffer3 05
dH2O 28.528.5
TOTAL 50μl50μl

-->left for 3 hours at 37 °C.

-->(1)20μl refine

-->(2)20μl refine