Team:Chiba/Calendar-Home/3 September 2008

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2 September 2008 <|> 4 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(2/9)--->Mini prep


--->Gel Check
Chiba-0903.JPG
Sample DNA 1
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
From left;
Chiba-0903-2.JPG
From left;
--->Digestion test
Digestion SingleDouble
Sample DNA(μL) 13
XbaⅠ(μL) 0.10.1
PstⅠ(μL) 0.10.1
Buffer 2(μL) 0.9-
Buffer 3(μL) --0.8
BSA(μL) 11
dH2O(μL) 75
TOTAL(μL) 1010


--->Gel Check
Chiba-0903-3.JPG
Sample DNA(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12
From left;
Chiba-0903-4.JPG
From left;


(2/9)--->Phenotype-test
  • MIX
  • BBa_K084007(Plac+RBS+LasI, Competent Cells : JW1908) -> Sample Name : L1~L4
  • BBa_K084008(Plac+RBS+RhlI, Competent Cells : JW1908) -> Sample Name : R1~R4
  • BBa_S03623(Ptet+RBS+LuxI, Competent Cells : JW1908)
  • BBa_T9002(Ptet+RBS+LuxR+GFP, Competent Cells : JW1908)


Sample No. 1234567891011
L1 2mL----------
L2-2mL---------
L3--2mL--------
L4---2mL-------
R1----1mL------
R2-----1mL-----
R3------1mL----
R4-------1mL---
BBa_S03154--------2mL--
AHL(100μM)----------1μL
BBa_T90022mL2mL2mL2mL1mL1mL1mL1mL2mL1mL1mL
IPTG(100μM)1μL1μL1μL1μL1μL1μL1μL1μL---
  • Incubated for 8hours at 37 degrees
  • Spindown (max rpm, 3 min)
  • measured the intensity GFP(visual judgment)
Sample No. 1234567891011
the intensity GFP +++++++++++++-


  • Left for 12 at room temperture.
  • Resuspensioned
  • measured the intensity of GFP by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6)
Sample No. 1234567891011
the intensity GFP 25.6518.9120.3921.8526.6226.5830.0733.6027.4110.7255.54

Team:Output

Digestion test

Sample No. 1~5
DNA tamplate(μL) 5
Buffer3(μL) 1
dH2O(μL) 4
EcoRI(μL) 0.1
TOTAL(μL) 10

-->37°C30min

-->Gel check

Sample No. 1~5
DNA(digested sample(μL)) 10
loading Dye(μL) 2
TOTAL(μL) 12