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From 2008.igem.org

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3 September 2008 <|> 5 September 2008

Contents 1 Laboratory work 1.1 Team:Input 1.2 Team:Communication 1.3 Team:Output

Laboratory work

Team:Input

no work

Team:Communication

--->Gel Check

Sample DNA 1 Loading Dye 1 dH2O 4 TOTAL 6μL From left; BBa_C0161(2007) -> OK BBa_C0161(2007) -> OK BBa_C0161(2006) -> OK BBa_C0161(2006) -> OK

From left; BBa_C0261(2007) -> OK BBa_C0261(2007) -> OK BBa_C0261(2006) -> OK BBa_C0261(2006) -> OK

--->Digestion test

Sample	Single Digesiton	Double Digestion
Sample DNA	1	2
EcoRI	0.05	0.05
PstI	-	0.05
Buffer 3	1	1
BSA	1	1
dH2O	6.95	5.9
TOTAL	10μL	10μL

--->Gel Check

Sample DNA 10 Loading Dye 2 TOTAL 12μL

From left; Single Digestion BBa_C0161(2007) BBa_C0161(2007) BBa_C0161(2006) BBa_C0161(2006) BBa_C0261(2007) BBa_C0261(2007) BBa_C0261(2006) BBa_C0261(2006) ladder Double digestion BBa_C0161(2007) BBa_C0161(2007) BBa_C0161(2006) BBa_C0161(2006) BBa_C0261(2007) BBa_C0261(2007) BBa_C0261(2006) BBa_C0261(2006)

Transformation

Competent cells : JW1908

Transformed the following and grew on new ampicillin plates.

• BBa_K084009(R-3,4,6,7)
• BBa_K084010(C-1,2,6,7)
• BBa_T9002(2007)

--->(5/9)Liquid Culture

Team:Output

Transformation

• BBa_J52008+BBa_F2620 x3
• BBa_K084003 x3

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