Team:Chiba/Calendar-Home/6 September 2008

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5 September 2008 <|> 7 September 2008

Contents

1 Meeting with UmeG
2 Laboratory work

Meeting with UmeG

the ppt (in Japanese) for our weekly meeting

File:Chiba-0809060855.ppt

Laboratory work

Team:Input

no work

Team:Communication

PCR of BBa_I9030 and two control,BBa_F2620(2007)).

DNA Template	1
dNTP mix	5
Foward Primer	5
Reverse Primer	5
DNA polymerase	1
Thermopol Buffer	5
dH₂O	33
TOTAL	50μL

95°C,5min -> ( 95°C,1min -> 54°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C store

--->Gel Check

Sample DNA	1
Loading Dye	1
dH₂O	4
TOTAL	6μl

From left;

1 BBa_I9030 without LVA -> OK

2 BBa_I9030 Positive Control -> OK

3 BBa_F2620 Positive control -> OK

Sample DNA : BBa_R0010

Sample DNA	1
Loading Dye	1
dH₂O	4
TOTAL	6μl

R0010 --> 100μL

Digestion Test

Sample DNA:

1:BBa_R0010,2:BBa_S03154,3:BBa_J04500,4:BBa_C0161,5:BBa_C0261

Sample No.	1	2	3	4	5
Sample DNA	1	1	1	1	1
PstI	0.5	0.5	0.5	0.5	0.5
SpeI	0.5	-	1	-	-
XbaI	-	0.5	-	0.5	0.5
Buffer 2	2	-	3	-	-
Buffer 3	-	6	-	6	6
(10×)BSA	2	0.6	3	-	-
(100×)BSA	-	-	-	0.6	0.6
dH₂O	3	6.4	7	1.4	1.4
TOTAL	20μl	60μl	30μl	60μl	60μl

Team:Output

no work

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