Team:Chiba/Calendar-Home/9 September 2008

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8 September 2008 <|> 10 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(6/9)--->PCR of BBa_I9030


--->Gel Extract

Chiba-0909-3.JPG
Sample DNA 60
Loading Dye 12
TOTAL 72μL
CinI


--->Zymo Clean

  • CinI
BBa_C0161
BBa_C0261
BBa_S03154
BBa_R0010


--->Gel Check

Chiba-0909.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μL
From left;
CinI -> ?
BBa_C0161 -> OK
BBa_C0261 -> OK
BBa_S03154 -> OK (low)
Marker
BBa_R0010 -> OK (low)
Chiba-0909-2.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μL
BBa_J04500 -> OK
  1. LuxI(BBa_K084012)
  2. LuxI+LVA(BBa_K084014)
  3. LasI(BBa_K084007)
  4. Background(R0010)
  5. Background(BBa_C0261)
--->Ligation
Sample No. (1)(2)(3)(4)(5)
insert-C0161 3----
insert-C0261 -2--2
insert-S03154 --6--
Vector-R0010 -555-
vector-J04500 3----
ligase 11111
Buffer 22422
dH2O 1-425
TOTAL 1010201010


---> Transformation

---> Colony Count( 10/9)

Team:Output

no work