Team:Chiba/jk/γ/time

From 2008.igem.org

Contents

Time Delay Test

Designs

In this plan, we induce cross-talk of quorum sensing by changing receiver protein.

Expetriments

  • sender
  • AHL sender(BBa_S03623)
  • reciever
  • LuxR
  • RhlR(BBa_K084004)
  • LasR(BBa_K084005)
  • CinR(BBa_K084006)
  • reporter
  • Plux-GFP(BBa_J37032)

Method

  1. Transformed Sender into E.coli strains(JW1908) and Receivers into E.coli strain(JW1908).
  2. Inoculated them independently in liquid media. Incubated at 37c° 12h.
  3. Inoculated again at 37c° upto about OD600=2.0
  4. Washed them.
  5. Mixed them (Sender:Receiver=1000μl:1000μl).
  6. Incubated at 30c°.
  7. Measured intensity of green fluorescence at regular time intervals.


Result

Fig.X-ATime Delay Test Sender:LuxI,Receiver:LuxR
Fig.X-BTime Delay Test Sender:LuxI, Receiver:LasR
Fig.X-CTime Delay Test Sender:LuxI, Receiver:RhlR


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