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Team:Edinburgh/bglX
From 2008.igem.org
Contents |
bglX
AMINO ACID SEQUENCE
MKKITVLISIWLSAAAFFSCEKKTEAGSKPQAFDKEVQDLLKNMSLEEKAGQMTQIDI
RNLLNNGYGNTDEKLDTARLKEAIQTYHVGSILNCIQAYTPEKWVELISQIQNEALQS
PNKIPVLYGTDAMHGVGFIKDAVLFPHNIGMAASRNDQLVSQAAQVTSTEARSVGLTW
NFAPVLDVGREPYWSRFEETFGEDVYITTQMGSAAVQMMEGSDLTSKTNIASCLKHFI
GYSAPKNGIDRTQSHIPEIVLREYYLPPFREAINKGASSIMINSAEINGIPCHGNKWL
LTDLLRTELGFTGMVVSDWEDVIRLHTWHKVAATPKEAVMMAVNAGVDMSMVPNDYSF
PKYLVELVKEGKVSMARIDEAVGRILTLKIKLGLMKNPLPSIADVGVVGSDAHQQIAL
NAARESITLLKNDKNILPLAKDKKILLVGPAANSLSALHSSWSYTWQGSNESLYPETT
KTIREALEASGNKANIRTNATTGFDDAANYDVSFIQKNTAGVDVIIVCVGEAAYAEQP
GVIKDLNLPEAQKQLIVAAKKTGKPVIVCLVEGRPRLFPEEEALADAVIMCYRPGSKG
ADAFAEILYGDINPSGKLPFTYPRYDGDITTYDYKFKETEQQLKPGVSEFVAFNPQWP
FGHGLSYTTFAYSNLNVNKSNFTKNDSVLVTVDISNTGARTGKIAVELYSRDHFASIT
PSERRLRKYTKIELKAGEKQTVSFTIKAADLQFVNKDLKTVTEAGAFDLMIGNLQTEI
YFNE
DNA SEQUENCE
atgaaaaaaataaccgtattgatttccatctggctcagtgcagccgcttt 50
tactttttttattggcataactaaaggtagaccgagtcacgtcggcgaaa
tttctcttgtgagaaaaaaacagaagcggggagtaaacctcaggcctttg 100
aaagagaacactctttttttgtcttcgcccctcatttggagtccggaaac
ataaagaagtacaggatctgttaaaaaatatgtcgctggaagaaaaagcc 150
tatttcttcatgtcctagacaattttttatacagcgaccttctttttcgg
ggacagatgacgcagatcgatatccgtaatttattgaacaacggatatgg 200
cctgtctactgcgtctagctataggcattaaataacttgttgcctatacc
aaataccgatgaaaaattagataccgccagattaaaagaagccattcaaa 250
tttatggctactttttaatctatggcggtctaattttcttcggtaagttt
cctatcatgtcggttctatattaaattgcattcaggcatacacacctgaa 300
ggatagtacagccaagatataatttaacgtaagtccgtatgtgtggactt
aaatgggttgaattgatttctcagatccagaacgaagcattgcaatcacc 350
tttacccaacttaactaaagagtctaggtcttgcttcgtaacgttagtgg
taataaaattccggttttatacggaacagatgccatgcatggtgttggct 400
attattttaaggccaaaatatgccttgtctacggtacgtaccacaaccga
ttattaaagatgccgtattgtttccgcataacataggtatggctgcatcc 450
aataatttctacggcataacaaaggcgtattgtatccataccgacgtagg
cgcaacgatcaattggtttcgcaggcagctcaggtaacgtcaacagaagc 500
gcgttgctagttaaccaaagcgtccgtcgagtccattgcagttgtcttcg
gcgttccgttggccttacctggaattttgctccggtgcttgacgtaggca 550
cgcaaggcaaccggaatggaccttaaaacgaggccacgaactgcatccgt
gagaaccttactggtcgcgctttgaagaaacctttggtgaagatgtatac 600
ctcttggaatgaccagcgcgaaacttctttggaaaccacttctacatatg
ataacaacacaaatgggttcagcagccgtacaaatgatggaaggcagtga 650
tattgttgtgtttacccaagtcgtcggcatgtttactaccttccgtcact
cctgacgtctaaaacaaacatagcttcctgcctgaaacactttattggat 700
ggactgcagattttgtttgtatcgaaggacggactttgtgaaataaccta
attcagcacccaaaaatggtatagaccgcacacaatcacacattcctgaa 750
taagtcgtgggtttttaccatatctggcgtgtgttagtgtgtaaggactt
attgtgctgcgcgaatattatctgcccccgttcagagaagcgatcaataa 800
taacacgacgcgcttataatagacgggggcaagtctcttcgctagttatt
aggcgcttcatccattatgatcaactctgctgagatcaatggcattccgt 850
tccgcgaagtaggtaatactagttgagacgactctagttaccgtaaggca
gtcacggcaacaaatggctgttaacagatctgctccgtacagagcttgga 900
cagtgccgttgtttaccgacaattgtctagacgaggcatgtctcgaacct
tttaccggaatggttgtatcggattgggaagatgtgatccgtttgcacac 950
aaatggccttaccaacatagcctaacccttctacactaggcaaacgtgtg
ctggcataaagtagctgctacgccgaaagaagccgtaatgatggccgtta 1000
gaccgtatttcatcgacgatgcggctttcttcggcattactaccggcaat
acgcaggtgtggatatgagcatggtgccgaatgattactcgtttccgaaa 1050
tgcgtccacacctatactcgtaccacggcttactaatgagcaaaggcttt
tatctggtagaattggtgaaagaaggaaaagtttctatggcgcgcatcga 1100
atagaccatcttaaccactttcttccttttcaaagataccgcgcgtagct
cgaagcggtgggacgtattctgaccctgaaaatcaaactgggcttgatga 1150
gcttcgccaccctgcataagactgggacttttagtttgacccgaactact
aaaatccattgccatccattgcagatgttggtgtagtgggttctgatgca 1200
ttttaggtaacggtaggtaacgtctacaaccacatcacccaagactacgt
catcaacagatcgcactaaatgcagcgcgtgaatcaattacattattgaa 1250
gtagttgtctagcgtgatttacgtcgcgcacttagttaatgtaataactt
gaacgataaaaatattctgcctcttgcaaaagataaaaaaatactattgg 1300
cttgctatttttataagacggagaacgttttctatttttttatgataacc
ttggtccggccgctaacagtttatctgcgctgcacagttcatggtcatac 1350
aaccaggccggcgattgtcaaatagacgcgacgtgtcaagtaccagtatg
acatggcagggaagcaatgaatcgctgtatcctgaaacaactaaaacaat 1400
tgtaccgtcccttcgttacttagcgacataggactttgttgattttgtta
ccgtgaggcattagaagcgagcggtaataaagctaacatcagaacgaatg 1450
ggcactccgtaatcttcgctcgccattatttcgattgtagtcttgcttac
cgactacaggatttgatgatgcagcaaattatgatgtttcctttattcaa 1500
gctgatgtcctaaactactacgtcgtttaatactacaaaggaaataagtt
aaaaatacagcaggggtggatgtaattattgtgtgtgtaggtgaagctgc 1550
tttttatgtcgtccccacctacattaataacacacacatccacttcgacg
ctatgccgaacagcctggagtaatcaaggatctcaatctgccggaagcgc 1600
gatacggcttgtcggacctcattagttcctagagttagacggccttcgcg
agaagcaattgattgttgctgcaaagaaaacaggcaaacctgtcattgta 1650
tcttcgttaactaacaacgacgtttcttttgtccgtttggacagtaacat
tgcctggtagagggcaggccgcgtttgtttccggaagaagaagcacttgc 1700
acggaccatctcccgtccggcgcaaacaaaggccttcttcttcgtgaacg
agatgctgtaataatgtgctaccgtccgggcagcaaaggtgcagacgcat 1750
tctacgacattattacacgatggcaggcccgtcgtttccacgtctgcgta
ttgcagagattctatacggtgacatcaatcccagcggtaagcttccgttc 1800
aacgtctctaagatatgccactgtagttagggtcgccattcgaaggcaag
acctatccgagatacgacggagatattactacatacgattataaatttaa 1850
tggataggctctatgctgcctctataatgatgtatgctaatatttaaatt
ggaaaccgagcagcagttaaaaccgggcgtgagtgaatttgttgcgttca 1900
cctttggctcgtcgtcaattttggcccgcactcacttaaacaacgcaagt
atccgcaatggccattcggacatggcttgagctacacaacgtttgcatat 1950
taggcgttaccggtaagcctgtaccgaactcgatgtgttgcaaacgtata
tcaaaccttaacgtgaacaaaagtaattttacgaagaacgatagtgtgct 2000
agtttggaattgcacttgttttcattaaaatgcttcttgctatcacacga
ggtaacggtagacataagcaatacaggagcacgtacaggtaagatagccg 2050
ccattgccatctgtattcgttatgtcctcgtgcatgtccattctatcggc
tagagttatattccagagaccacttcgcatcgattacgccttctgaaaga 2100
atctcaatataaggtctctggtgaagcgtagctaatgcggaagactttct
cgcttacgtaaatacacaaagattgaactgaaagcaggagagaagcaaac 2150
gcgaatgcatttatgtgtttctaacttgactttcgtcctctcttcgtttg
cgtttcgtttacgatcaaagcagcggatctgcaatttgtaaataaagatc 2200
gcaaagcaaatgctagtttcgtcgcctagacgttaaacatttatttctag
tgaaaacagtaaccgaagcaggcgcttttgatctgatgatcggaaatctc 2250
acttttgtcattggcttcgtccgcgaaaactagactactagcctttagag
cagacagaaatatattttaatgagtaa 2277
gtctgtctttatataaaattactcatt
Forbidden Restriction Sites: None!
BIOBRICKTM PRIMERS
Forward: CGT gaattc gcggccgc t tctagATG AAA AAA ATA ACC GTA TTG
Reverse: CGT t actagt a TTA TTA CTC ATT AAA ATA TAT TTC TG
