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- Cyanobacteria are photosynthetic bacteria that can convert carbon dioxide to sugars. The main goal of the Hawaii team is to make a "toolkit" for cyanobacteria, which hold promise as producers of biofuels and other bioproducts.
- We constructed a number of tools to more easily and effectively engineer cyanobacteria, including
- 1) a BioBrick switch that turns on a downstream gene when nitrate is added,
- 2) a BioBrick that mediates protein export from the cell into the surrounding medium and
- 3) a naturally occurring shuttle vector to BioBrick format to allow easy transfer of existing and new BioBricks into cyanobacteria.
- Our toolkit was designed to allow controlled production and recovery of bioproducts. Additionally, our vector has the potential to transform other gram negative bacteria and possibly even plants via Agrobacterium.