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Team:Hawaii/Notebook/2008-06-12
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Triparental conjugation
- Grace
- Began triparental conjugation between DH5α transformed with pRL1383a, E. coli with RP1, and PCC6803 (see experiment)
- Innoculated BG-11 + 5% LB plates
Purified BBa_B0034 colonies
- Krystle
- Streaked out colonies onto fresh LB+amp100 plates
Preparation for making competent cells
- Krystle & Grace
- Autoclaved detergent-free erlenmeyer flasks
- Innoculated 250 ml SOB w/ DH5α cryostock; shook overnight at 26C with shaking (150 rpm)
Made BG-11+sm+sp plates
- Krystle
- Made 20 plates
- 2.5 μg/ml each of sm and sp, labeled Sm2.5Sp2.5
Made SOB media
- Krystle
- 2 batches (and added antibiotic... oops! will autoclave again to get rid of it)
Made BG-11
- Norman
- stored in room temp incubator bottom shelf
- will need to make bunch of 50 mL stock solution of TES and Thisulfate during some downtime.
Drylab Work
Sequence synthesis
- Grace
- rbc promoter Biobrick sequence to be synthesized
Parts list
- Norman
- Parts list submitted by team members; Norman will send a request to iGEM for parts not on filter paper
Primer Design Review
- Norman
- will review some primers tonight before shelling out money to buy'm
Discussion
Quote of the Day
- "We like lab math, except when we have to divide by even numbers. We can't divide by even numbers therefore we can't do binary fissions." -GK, KS
- "Bye" -GK, KS in synchronized tone...
- "You girls should join a choir..." -GP
- "You not only dress the same, you talk with the same voice at the same time, are you trying to confuse me again!?" -NW
