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Team:Hawaii/Notebook/2008-06-13
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Prepped Cells for Glycerol Stock/Seed Stock
- Krystle
- Inoculated a single colony in each 10 mL LB + antibiotic
- Inoculated a single colony in each 10 mL LB + antibiotic
| Antibiotic | Cell type | Plasmid |
|---|---|---|
| Amp100 | StrataClone supercompetent cells | pUC18 |
| DH5α | GFP | |
| DH5α | tet+GFP | |
| DH5α | RBS (BBa_B0034) | |
| Kan50 | HB101 | RK1 |
| Sm2.5 + Sp 2.5 | DH5α | pRL1383a |
Prepped Cells for Competency
- Grace
- Checked yesterday's inoculation. OD600=1.2 (too high). Need to redo.
- Made SOB for new inoculation.
- Inoculated 250 μl SOB with 100 μl DH5α. Inoculated 250 μl SOB with 10 μl DH5α. Incubated overnight at 27C with shaking (150 rpm).
Cell density test
- Grace
- Retrieved plates from SC incubator. All plates had too many colonies (lawn). Need to redo.
- Made BG-11 plates for retest next week.
Made LB agar stock
- Grace
- (2) 300 mL batches of LB + 1.5% Agar. Stored in 4C.
Discussion
- We need to make more BG-ll 50x stock solution and TES 50x stock solution
- Kelly from SC lab will be in 12pm-3pm. We should retrieve our triparental conjugation plates then.
- Grace, Krystle, and Norman will come in at 9am tomorrow to plate triparental conjugates, make competent cells, and glycerol stock purified Biobrick colonies.
Quote of the Day
