Team:Hawaii/Notebook/2008-06- 9

From 2008.igem.org

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Things we did today

Wetlab Work

Practiced getting DNA plasmids off the filter paper

Norman and Krystle
  • see experiment for details
DNA will be frozen in TE for later transformation
BBa_E0040 GFP biobrick (spot 7E on plate 1001)
BBa_J04430 IPTG-induced GFP device (spot 4H on plate 1004)
BBa_I13522 constitutive GFP device (spot 8A on plate 1002)

Made Spectinomycin/Streptomycin Plates

Grace
  • Made 1000X sp stock solution
  • Made 1000X sm stock solution
  • Made BG-11+sp+sm plates
  • Made LB+sp+sm plates
  • Made LB+sp plates

Transformed BioBricks into competent DH5α

Grace

Drylab Work

Discussion

Quote of the Day

"So that's the difference between biochemists and geneticists...
Biochemists will be so meticulous and do everything the long way.
Geneticists will try to look for a shortest, simplest possible."
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