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Team:Hawaii/Notebook/2008-07- 1
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
PCR gel
- Margaret
- Ran 1% gel for oriV, rep, mob, and omega PCR products
Glycerol stocked Biobricks
- Grace
- Glycerol stocked B0030, C0012, E0040, J33207, and R0010
Made LB liquid media
- Grace
- Two 750ml batches
Plasmid prep
- Grace
- Took 1 ml of the overnight Biobrick cultures to inoculate 100 ml LB+amp100. Incubated 8 hours at 37C with shaking (150 rpm). Stored in 4C until tomorrow morning.
Biobrick extraction
- Grace
- B0014 (double terminator), plated on LB+amp50+kan20
- B0015 (double terminator), plated on LB+amp50+kan20
- E0026 (enhanced cyan fluorescent protein), plated on LB+amp100
- E0240 (GFP device), plated on LB+amp100
- I14032 (lac promoter), plated on LB+kan50
- J04430 (IPTG induced GFP device), plated on LB+amp100
Drylab Work
Project description write-ups
- Margaret & Grace
- Updated project descriptions on wiki
Secretion project presentation
- Krystle & Grace
- Worked on powerpoint and flowchart for Thursday's meeting
Plans for the rest of the week
- Margaret, Krystle, Grace, and Norman
- Tomorrow
- Redo PCR and run on gel (MR)
- Plasmid prep for B0030, C0012, E0040, J33207, and R0010 (MR)
- Restock cyanos (KS)
- Restreak any transformed Biobrick plates from today (GK)
- Thursday
- Organize cryostocks (GK)
Discussion
- FYI: Spectrophotometer broken. Make arrangements to use someone else's spectrophotometer if needed.
Quote of the Day
GK: "It depends on if we're dinnering tomorrow."
KS: "Since when did dinner become a verb?"
