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Things we did today

Wetlab work

Checked DNA products via gel electrophoresis

EtBr stained 2% agarose gel ran at 72V for 100 min. Twenty five microliters of the RE digest reactions were loaded into each lane.

Grace

• Ran RE digests on an EtBr stained 2% agarose gel at 72V for 100 min.

• Picture really dark. Adjust camera? Restain with more EtBr tomorrow?

• Resuspended plasmid preps from yesterday in 50 μl TE buffer and ran 5 μl on an EtBr stained 1.2% agarose gel at 95V for 1 hour

• Plasmid prep for I14032 (2008 distribution) was discarded. Solution was very thick (like egg whites) during resuspension, indicating high levels of proteins present.
• Plasmids didn't run. Still really close up to to wells. Trouble shoot tomorrow.

Cryostocks

Grace

• E. coli did not grow. Reinoculate tomorrow.

Discussion

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