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Team:Hawaii/Notebook/2008-08-20
From 2008.igem.org
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Things we did today
Wetlab work
Name of Task
- Margaret & Krystle
- Annealed and phosphorylated:
- plac (I14032) for front ligation
- rbs 1.0 (B0034) for back ligation
- rbs 0.6 (B0030) for back ligation
- rbs 0.6 (B0030) for front ligation
- tt (B1006) for back ligation
- We decided to perform this reaction again with out gel purification and the gel was discarded.
- These annealed products can be found in the -20°C freezer in the DNA box.
- Quantification was achieved simply by adding up the amount of DNA added.
- plac (I14032) for front ligation --> 370ng/ul
- rbs 1.0 (B0034) for back ligation --> 206.7ng/ul
- rbs 0.6 (B0030) for front ligation -->
- rbs 0.6 (B0030) for back ligation --> 228.3ng/ul
- tt (B1006) for back ligation --> 375ng/ul
Restriction Digest
- Krystle
- B0034 with EcoRI and XbaI (for ligation with plac)
- nir with SpeI and PstI (for ligation with RBS)
- gfp wih SpeI and PstI (for ligation with TT)
- gfp with EcoRI and XbaI (for ligation with RBS)
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
