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Team:Hawaii/Notebook/2008-09-26
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of Transformants
- Grace
| Construct | Colonies |
|---|---|
| nir+rbs+GFP + tt | 3 |
| nir + rbs+GFP+tt1 | 1 |
| plac + rbs+GFP+tt1 | 1 |
| nir + rbs+GFP+tt2 | 5 |
| plac + rbs+GFP+tt2 | 0 |
| pSB1A3 to self (- control) | 0 |
| J33207 + pRL1383a (plated on IPTG+Xgal) | 31 |
| J33207 + pRL1383a (1:10) (plated on Xgal) | 0 |
| pRL1383a to self (+ control) | 0 |
| J33207 plasmid (+ control, plated on IPTG+Xgal) | 162 (all blue!) |
| pRL1383a plasmid (+ control) | 0 |
| no DNA | 0 |
- Colony PCR of transformants
- Bands too small for all secretion constructs
- BB-pRL1383a bands all ~875 (expected size = 850bp). =D!
- Restreaked BB-pRL1383a colonies #1, 3, 13, 18
Drylab Work
iGEM presentation
- Margaret, Krystle, Grace
- Reviewed Margaret's presentation for Dr. Lee and outlined presentation for Boston.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
