"
Team:Hawaii/Notebook/2008-09-30
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid Prep
- Grace
- Plasmid prep of BBpRL1383a #1, 3, 13, 18
Construction of secretion device (cont.)
- Grace
- PCR:
- nir+rbs
- slr1+GFPf
- plac+rbs
- rbs+GFP
- nir
- nir+rbs+pilA #17 (yet to be confirmed via sequencing)
- rbs+GFPf+tt #1, 2 (yet to be confirmed via sequencing)
- GFPf+tt #21
- Overnight RE digest:
- EcoRI/PstI:
- pSB1A3
- EcoRI/SpeI:
- nir
- rbs+GFP
- nir+rbs
- plac+rbs
- slr1+GFPf
- nir+rbs+pilA #17
- XbaI/PstI:
- slr1+GFPf
- rbs+GFPf+tt #1, 2
- GFPf+tt #21
- rbs+GFP
Plasmid Prep
- Margaret
- pSB1A3, plac/rbs/rep + pSB1A3 (also made a glycerol stock of this), and oriT in pSB1A3
RE-digest from yesterday
- Margaret
File:Gel purification 9-30.jpg
Gel purification of re-digest of aadaBB, aadapRL, and oriV.
- gel purification of aadaBB, aadApRL, and oriV
- de-phosphorylate pSB1A2, pSB1A3a, pSB3K3
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
