Team:Hawaii/Notebook/2008-09- 8

From 2008.igem.org

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Things we did today

Wetlab work

Inoculated for plasmid prep and cryostock

Grace
  • nir+rbs
  • rbs+GFP
  • plac+rbs
  • slr1+GFPf
  • pilA+GFPf (8/12 and 8/13 transformations)

Restreaked to purify colonies

Grace
  • 3A: plac+rbs colony #10
  • BB-pRL plates

PCR

Krystle
  • 20ul reactions of nir, pilA, slr1 using VF2 and VR primers
  • 10ul reaction plac +rbs using VF2 and rbs specific primer

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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