Team:Hawaii/Notebook/2008-09- 8
From 2008.igem.org
Projects | Events | Resources | ||
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Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Inoculated for plasmid prep and cryostock
- Grace
- nir+rbs
- rbs+GFP
- plac+rbs
- slr1+GFPf
- pilA+GFPf (8/12 and 8/13 transformations)
Restreaked to purify colonies
- Grace
- 3A: plac+rbs colony #10
- BB-pRL plates
PCR
- Krystle
- 20ul reactions of nir, pilA, slr1 using VF2 and VR primers
- 10ul reaction plac +rbs using VF2 and rbs specific primer
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson