Team:Hawaii/Notebook/2008-09- 9

From 2008.igem.org

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Things we did today

Wetlab work

Cryostock

Grace
  • nir+rbs
  • rbs+GFP

Plasmid prep

Grace
  • nir+rbs
  • rbs+GFP
  • plac+rbs
  • slr1+GFPf
  • pilA+GFPf (8/12 and 8/13 transformations)

PCR

Grace
  • nir+rbs
  • rbs+GFP
  • plac+rbs
  • slr1+GFPf
  • pilA+GFPf (8/12 and 8/13 transformations)
  • slr1
  • pilA

Verified Krystle's PCR products from yesterday

Grace
EtBr stained 2% agarose gel ran at 95V for 1 hour. One microliter of PCR reaction were loaded into each well.

RE digest

Grace
  • EcoRI and SpeI:
  • nir + rbs
  • plac + rbs
  • XbaI and PstI:
  • slr1
  • pilA
  • slr1 + GFPf
  • pilA + GFPf (8/12)
  • pilA + GFPf (8/13)
  • EcoRI and PstI:
  • pSB1A3

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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