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Team:Hawaii/Notebook/2008-10-15
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of secretion device (cont.)
- Grace
- Transformed DH5α
- Used 5 μl of n/p + rgt ligation reaction
- Used 1 μl of J33207/pRL1383a ligation reaction
- Plated 25 μl, 50 μl, 75 μl, and 100 μl plates in hopes of isolating single colonies
- Krystle
- PCR
- nir+rbs
- slr+gfpf
- Restriction digest overnight
- nir+rbs with EcoRI and SpeI
- slr+gfpf with XbaI and PstI
- pSB1A3 with EcoRI and SpeI
Ligation
- Margaret
- lac promoter/rbs(34) (E,S) + rep2 (X,P) + pSB1A3 (de-phosphorylated, E,P)
- lac promoter/rbs(30)rep/oriV(6) (E,S) + lac promoter/rbs(34)aada/oriT(A) (X,P) +pSB1A3 (de-phosphorylated, E,P)
- lac promoter/rbs(30)rep/oriV(6) + lac promoter/rbs(34)aada/oriT (A)
- lac promoter/rbs(30)rep/oriV (6) (E,S) + lac promoter/rbs(34)aada/oriT(B) (X,P) +pSB1A3(de-phosphorylated, E,P)
- oriV (E,S) + oriT (X,P) + pSB1A7 (E,P)
- pSB1A7 self (did not de-phosphorylate) (E,P)
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
