From 2008.igem.org

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Things we did today

Wetlab work

Plasmid prep of BB-pRL1383a

Grace

• E. coli did not grow. Reinoculated.

Extracted J04430 from filter paper

Grace

EtBr stained 2% agarose gel ran at 95V for 1 hour. Ten microliters of PCR product were loaded.

• Used 2 μl of filter paper solution for PCR to verify plasmid

• Something is wrong with the J04430 filter spot iGEM sent us. PCR with VF2/VR primers did NOT yield the expected band ~1000bp.

Transformation

Grace

• Used 2 μl each of BBpRL1383a-1 and J04430 (from filter) to transform DB3.1

Construction of GFP secretion device

Krystle

• Gel purify restriction digests from yesterday
• Ligate nir+rbs and slr+gfpf into restricted psB1A3 from Margaret
• Transformed into DH5α

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

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