Team:Hawaii/Notebook/2008-10-29

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Things we did today

Wetlab work

Antibiotic Test, round 2

Grace
EtBr stained 2% agarose gel ran at 95V for 1 hour. Three microliters of PCR product were loaded into each lane.
Colony Count of J33207+pRL1383a Transformants
Plate Cells Colonies Blue?
LB+Amp100X-gal
(+) control 		J33207 in pSB1A2 in DH5α too many to count, medium yes
LB+sp100X-gal
(+) control 		BBpRL1383a-1
(10/25 inoculation plasmid prep) in DH5α 		too many to count, small no
LB+X-gal
(+) control 		untransformed DH5α lawn/smear no
LB+sp2.5+X-gal
(-) control 		untransformed DH5α 0 no
LB+sp100
(-) control 		untransformed DH5α 0 no
LB+sp2.5 J33207+pRL1383a ligation in DH5α lawn/smear no
LB+sp5 J33207+pRL1383a ligation in DH5α lawn/smear no
LB+sp10 J33207+pRL1383a ligation in DH5α lawn/smear no
LB+sp20 J33207+pRL1383a ligation in DH5α lawn/smear no
LB+sp25 J33207+pRL1383a ligation in DH5α lawn/smear no
LB+sp40 J33207+pRL1383a ligation in DH5α too many to count, small no
LB+sp50 J33207+pRL1383a ligation in DH5α too many to count, small to medium no
LB+sp75 J33207+pRL1383a ligation in DH5α too many to count, small to medium no
LB+sp100 J33207+pRL1383a ligation in DH5α too many to count, small to medium no
  • Colony PCR of J33207, BB-1, and sp75, 100transformants

Drylab Work

Project page

Grace
  • Updated materials, methods and results for Project Part B.


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


Sponsor_UHM.gifSponsor_OVCRGE.gifSponsor_CTAHR.gif


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