Team:Hawaii/Notebook/2008-10- 1

From 2008.igem.org

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Things we did today

Wetlab work

Construction of secretion device (cont.)

Grace
EtBr stained 2% agarose gel ran at 60V for 2 hours. Thirty microliters of RE digested product were loaded into each well.
  • Ran RE digest products on gel
  • nrp #17 band did not cut
  • plac+rbs band not visible on gel
  • GFPf+tt #21 band did not cut
  • Extracted bands from gel
  • Ligated:
  • nir & rbs+GFP
  • nir+rbs & slr1+GFPf
  • slr1+GFPf & tt
  • rbs+GFP & tt
  • nir & rbs+GFP+tt #1
  • plac & rbs+GFP+tt #1
  • nir & rbs+GFP+tt #2
  • plac & rbs+GFP+tt #2
  • plac & rbs+GFP
  • rbs + slr1+GFPf
  • pSB1A3 to self (control)
  • SAP treated pSB1A3 to self (to test for SAP/buffer contamination)
  • Used 5 μl ligation reaction to transform DH5α cells

Made LB+Amp100 plates

Grace

Made Amp100 1000X stock solution

Grace

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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