Team:Hawaii/Notebook/2008-10- 1
From 2008.igem.org
Projects | Events | Resources | ||
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Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of secretion device (cont.)
- Grace
- Ran RE digest products on gel
- nrp #17 band did not cut
- plac+rbs band not visible on gel
- GFPf+tt #21 band did not cut
- Extracted bands from gel
- Ligated:
- nir & rbs+GFP
- nir+rbs & slr1+GFPf
- slr1+GFPf & tt
- rbs+GFP & tt
- nir & rbs+GFP+tt #1
- plac & rbs+GFP+tt #1
- nir & rbs+GFP+tt #2
- plac & rbs+GFP+tt #2
- plac & rbs+GFP
- rbs + slr1+GFPf
- pSB1A3 to self (control)
- SAP treated pSB1A3 to self (to test for SAP/buffer contamination)
- Used 5 μl ligation reaction to transform DH5α cells
Made LB+Amp100 plates
- Grace
Made Amp100 1000X stock solution
- Grace
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson