From 2008.igem.org

Birds Eye  View Team	Projects	Events	Resources
Sponsors	Experiments	Milestones	Protocols
	Notebook (t)	Meetings (t)

Things we did today

Wetlab work

Construction of secretion device (cont.)

Grace

EtBr stained 2% agarose gel ran at 60V for 150 minutes. Thirty microliters of RE digest reaction were loaded into each well.

• Ran RE digests on gel

• J33207 did not cut

• Extracted bands from gel
• Ligated:

• nir + rbs+gfp+tt#1
• plac + rbs+gfp+tt#1
• nir+rbs + slr1+GFPf
• plac+rbs + slr1+GFPf
• nir+rbs + pilA+GFPf+tt
• plac+rbs + pilA+GFPf+tt
• pSB1A3 to self (control)

• Used 5 μl ligation mixture to transform DH5α cells

Drylab Work

Grace, Krystle, Margaret

• Revised project abstract

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers