Team:Hawaii/Parts

From 2008.igem.org

Contents

Guidelines for naming parts

Part number Part type
K125000-K125099 plasmid
K125100-K125199 promoter
K125200-K125299 ribosome binding site
K125300-K125499 signal sequences, conjugation sequences, random stuff
K125500-K125699 protein coding
K125700-K125799 transcription terminator
K125800-K125999 devices

Parts planned

Parts in process (building)

Part number Description Type
K125010 Broad-Host-Range plasmid derived from RSF1010 plasmid
K125330 P1 lytic replication
K125340 oriV
K125510 Plasmid replication proteins protein coding
K125520 aadA gene protein coding
K125800 rep proteins with promoter and rbs protein coding
K125830 Synechocystis-specific, nitrate-inducible GFP secretion device under the control of a nirA promoter (K125100+B0030K125310+K125500) device

Parts submitted

Part number Description Type
K125000 pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (lac promoter+lacZ) plasmid
K125100 nitrate inducible nir promoter regulator
K125110 nitrate inducible nir promoter + ribosome binding site with efficiency of 0.6 relative to B0030 regulator
K125300 cyanobacterial pilA derived amino termninus protein secretion signal sequence protein coding
K125310 cyanobacterial slr2016 derived amino terminus protein secretion signal sequence protein coding
K125500 GFP fusion protein protein coding
K125810 N-terminal slr2016 signal sequence attached to GFP fusion protein (Composite of K125310+K125500) protein coding
K125820 Synechocystis-specific constitutive GFP secretion device under the control of a lac promoter (I14032+B0034K125310+K125500) device
K125350 oriTr/LacZ conjugation screening
K125360 oriT/LacZ conjugation screening
K125320 oriT derived from RP4 origin of transfer
K125020 rep/oriv/aada/orit intermediate for RSF1010 derived plasmid
K125530 aadA gene with promoter and rbs protein coding