Team:KULeuven/25 August 2008

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Contents

Lab Work

Gel of the PCR products of the transduction

Wet Lab

We did a PCR to test the transduction with donor and acceptor as control. The PCR worked better then the previous time, but we couldn't see anything of the donor or acceptor (see picture). So we will have to do it again.

Ligations were electroporated (C0060+B0015, C0040+B0015, C0012+B0015, K145015+B0015, K145001+B0015 and R0040+J23022).

The following parts were miniprepped and digested: J23116+B0032, R0040+B0032, R0084+B0032 and R0040+J23022 (cut with EcoRI and SpeI) and R0011+F1610 (cut with XbaI).

The following ligations were set up: K145001+pSB1A2 and K145151+pSB1A2.

Dry Lab

Modeling

Further attempts at stochastic modeling of the filter using Matlabs SSA.

Remarks

Looking for a place to stay in New York ;)

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