Team:KULeuven/27 August 2008

From 2008.igem.org

• Home
• Reports
• Calendar

• Project abstract
• Meet the team
• Leave a message
• Mail us

• The Team
  Research Labs and Groups Students Instructors Advisors Pictures
• The Project
  Summary Components

  Input Output Filter InverTimer Reset Cell Death Memory

  Safety End Evaluation Literature Brainstorm
• Ethics
  Introduction Three A's

  Defining the three A's Developments in modern physics Developments in biology

  Concerns & Issues

  Defining synthetic biology A framework Concerns Issues The need for answers

  Biological Robotics

  KULeuven iGEM 2008 project Biological robotics

  Afterword References
• Submitted Parts
  Sandbox
• Modeling
  Overview Kinetic Constants Components

  Output Filter InverTimer Reset Cell Death Memory

  Full Model Sensitivity Analysis Multi-cell Model Diffusion
• Data Analysis
  Overview New Parts

  GFP (LVA-tag) Other

  Components

  Input Output Other
• Software
  Multi-cell Toolbox Simbiology2LaTeX Toolbox
• Notebook
  Protocols Reports

  Daily Weekly

  Lab Data

  Freezer Primers Ligation

  Tools Press Acknowledgments

Lab Work

Wet Lab

We continued our ligations. Therefore we miniprepped and digested the following parts: C0040+B0015, C0012+B0015, K145015+B0015, K145001+B0015, K145151+B0015 (with XbaI) and R0040+J23022 (with EcoRI and SpeI). However, when we put the digests on an agarose gel, we didn't see anything. But, according to the nanodrop, there should be DNA in our samples. So we put the miniprepped plasmids on gel (not digested) and we couldn't see anything - again. This means that either the miniprep failed or the colonies were crap. Tomorrow we will electroporate the ligated parts again (but we will plate them on Ap instead of Km). We will also redo the ligations, but this time we will use the protein coding regions as vector and B0015 as insert. This means cutting the vectors with SpeI and PstI and B0015 with XbaI and PstI.

Modelling has shown that our original setup requires some (major) adaptations. We miniprepped and digested some of the parts needed for these changes: B0033, B0034 and C0012. We also did this for R0011 and F1610, but F1610 seemed to be degraded - again.

There were colonies on the plates with K145001+pSB1A2 and K145151+pSB1A2. Liquid cultures were made.

We will make a glycerol stock of the ligations that succeeded. Therefore, we made a liquid culture of J23116+B0032, R0040+B0032, I712074+J23032, J23109+J23032and R0040+J23022.

Dry Lab

Ethics

Worked some more on the ethics-part. Did some more research.

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers