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Team:KULeuven/30 August 2008
From 2008.igem.org
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Lab Work
Wet Lab
A glycerol stock was made of the new parts and of our own parts (K145151, K145001, K145015). We forgot one, so a liquid culture was made of part R0053.
We miniprepped the new parts and the two ligations that succeeded (C0040+B0015 and C0060+B0015 that were plated on Ap instead of Km). They were then cut with XbaI.
The digests with XbaI were started: B0015, B0033, B0034, J23066, P0353, P0152, E0240.
The ligations we made yesterday were electroporated today (still in electrocompetent Top10 cells): R0011+B0032, C0040+B0015, C0060+B0015, C0012+B0015, K145015+B0015, K145001+B0015, K145151+B0015 and (R0040+J23022)+(J23109+J23032).
Dry Lab
General
Created the oligos for the B1002 and B1006 terminators link
Modeling
Final updated model uploaded to the wiki (only MatLab)
Remarks
I don't think the dam methylation is the problem with our ligations. This is the prefix: gaattcgcggccgcttctagag. The recognition site of XbaI is in bold. There is no overlap for dam methylation (gatc).
