We have been searching for an easy accessible output system, that could provide us qualitative and quantitative information in a quick and easy way. The choice for GFP was easily made.
A GFP with a C-terminal LVA tag for rapid degradation of the protein (Andersen et al, 1998) was chosen so that we can follow the output signal quickly in time. The Tet promoter (BBa_R0040) behaves as described in the Input section. For fine-tuning reasons, the RBS was chosen BBa_B0032 with a relative efficiency of 0,3.
This is a very easy system. As the Input results in inactivation of the tetR repressor, there is GFP production, proportional to the input signal.
Appl Environ Microbiol. 1998 Jun;64(6):2240-6. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S. PMID: 9603842