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Team:Michigan/24 October 2008
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October 24, 2008
Digested
pClock2 + GFP (1:3 ratio culture) and pClock2 + GFP (1:5 ratio culture) with EcoRI and PstI.
Started sequential
digest
of BioBrick GFP to cut with EcoRI and XbaI.
PCR of NifHp and NifL was
purified
.
Minipreped
samples of pLeuLP, pArabLP, GFP and pTRTp.
Transformed
pKD46 cells to electroporate to put pClock2 into the chromosome.
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