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Team:Mississippi State/12 August 2008
From 2008.igem.org
- redigest Lip and pPIC6 with EcoRI and NotI
- Prepare ligation mix
- prepare PCR mix to make more of the gene stock
- prepare and run 1% agarose gel
- purify PCR product
- digest pcr w/ notI and ecoRI
- cut out gel and purify w/ qiagen kit
- make overnight culture (xlI-blue)
- prepare ligation mix of digested Lip and pPIC6
